Metatranscriptomics reveals overall active bacterial composition in caries lesions

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Metatranscriptomics reveals overall active bacterial composition in caries lesions

BACKGROUND Identifying the microbial species in caries lesions is instrumental to determine the etiology of dental caries. However, a significant proportion of bacteria in carious lesions have not been cultured, and the use of molecular methods has been limited to DNA-based approaches, which detect both active and inactive or dead microorganisms. OBJECTIVE To identify the RNA-based, metabolic...

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Metatranscriptomics Reveals the Active Bacterial and Eukaryotic Fibrolytic Communities in the Rumen of Dairy Cow Fed a Mixed Diet

Ruminants have a unique ability to derive energy from the degradation of plant polysaccharides through the activity of the rumen microbiota. Although this process is well studied in vitro, knowledge gaps remain regarding the relative contribution of the microbiota members and enzymes in vivo. The present study used RNA-sequencing to reveal both the expression of genes encoding carbohydrate-acti...

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Spectroscopic Detection of Caries Lesions

Background. A caries lesion causes changes in the optical properties of the affected tissue. Currently a caries lesion can be detected only at a relatively late stage of development. Caries diagnosis also suffers from high interobserver variance. Methods. This is a pilot study to test the suitability of an optical diffuse reflectance spectroscopy for caries diagnosis. Reflectance visible/near-i...

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Bacterial composition in the supragingival plaques of children with and without dental caries.

PURPOSE The purpose of the present investigation was to determine if the supragingival bacterial composition plaques in children with caries would differ from those found in caries-free controls. MATERIAL AND METHODS Pooled supragingival plaque samples from the smooth surfaces of teeth were collected from 75 children with caries and 131 children without caries. The plaque samples were analyse...

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Characteristics of Early Caries Lesions in Enamel

The enamel demineralization defect has a lower mineral distribution in the surface layer in comparison to the adjacent sound enamel and also a lower interprismatic mineral content. The first stage of enamel demineralization is characterized by removal of interprismatic mineral content and in the subsequent stages a well defined surface layer formation occurs which constitutes early caries lesio...

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ژورنال

عنوان ژورنال: Journal of Oral Microbiology

سال: 2014

ISSN: 2000-2297

DOI: 10.3402/jom.v6.25443